Construction of a survivin promoter-mediated adeno-associated virus expression vector carrying a fusion gene constituted with cytosine deaminase and survivin mutant
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چکیده
منابع مشابه
Adeno-associated virus-mediated survivin mutant Thr34Ala cooperates with oxaliplatin to inhibit tumor growth and angiogenesis in colon cancer.
Colon cancer is one of the most common cancers. Survivin is overexpressed in human colon cancer and correlate with chemoresistance, angiogenesis and poor prognosis. Oxaliplatin, a platinum derivative cancer drug, has been used for treating human colorectal cancers. In the present study, we investigated the effect of the adeno-associated virus (AAV)-mediated survivin mutant Thr34Ala [rAAV-Sur-Mu...
متن کاملActivation of cancer-specific gene expression by the survivin promoter.
BACKGROUND Survivin, a member of the IAP (inhibitor of apoptosis) gene family, appears to be overexpressed in common cancers but not in corresponding normal adult tissues. To investigate whether the survivin promoter controls cancer cell-specific gene expression, we determined whether the survivin gene promoter could regulate reporter gene expression in cancer cell lines and xenografts. METHO...
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We have constructed a replication-deficient adenovirus encoding a nonphosphorylatable Thr(34)-->Ala mutant of the apoptosis inhibitor survivin (pAd-T34A) to target tumor cell viability in vitro and in vivo. Infection with pAd-T34A caused spontaneous apoptosis in cell lines of breast, cervical, prostate, lung, and colorectal cancer. In contrast, pAd-T34A did not affect cell viability of prolifer...
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An adeno-associated virus vector was constructed to express exogenous genes to the l iver. The original plasmid construct carried two expression units; a neomycin resistant gene and human 1antitrypsin cDNA under the control of hepatocyte specific transcription elements. Cel ls were transfected with the constructed plasmid DNA with another packaging plasmid, and recombinant adeno-associated viru...
متن کاملconstruction of vector fields with positive lyapunov exponents
in this thesis our aim is to construct vector field in r3 for which the corresponding one-dimensional maps have certain discontinuities. two kinds of vector fields are considered, the first the lorenz vector field, and the second originally introced here. the latter have chaotic behavior and motivate a class of one-parameter families of maps which have positive lyapunov exponents for an open in...
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ژورنال
عنوان ژورنال: World Chinese Journal of Digestology
سال: 2009
ISSN: 2219-2859
DOI: 10.11569/wcjd.v17.i11.1085